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IVF AND IUI

What do the grades on my embryo report actually mean?

You were handed a sheet of codes: 2PN, 8-cell, 4AA, arrested. It is your embryos' story, and you have every right to understand it.

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Reviewed by Dr Vani Sundarapandian, MD, DGO, FRCOG (UK). Last reviewed 29 September 2026.

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Key takeaways

  • An embryo grade describes how an embryo looked on one day; it is not a genetic test.
  • In a blastocyst grade like 4AA, the number is expansion, the first letter the inner cell mass, and the second the outer layer.
  • Grades help decide which embryo to transfer first; a lower-grade embryo can still become a healthy baby, and a high-grade embryo can fail.
  • Labs use different scales, so read your report against its own key, not someone else's.

The short answer

An embryo report records how each embryo looked under the microscope on each day. In a blastocyst grade such as 4AA, the number is expansion and the letters rate the inner cell mass and the outer layer. At Jananam Fertility Centre, Chennai, we read reports line by line and explain that a grade ranks embryos but never guarantees pregnancy.

01

What is on an embryo report?

A day-by-day record of your eggs and embryos, from egg collection to transfer or freezing.

Day 0 lists how many eggs were collected and how many were mature. Day 1 records which eggs fertilised normally. Days 2 and 3 count the cells in each embryo. Days 5 and 6 grade the blastocysts. The last lines say which embryo was transferred, which were frozen, and which stopped growing.

02

What does a blastocyst grade like 4AA or 3BB mean?

The number says how far the blastocyst has expanded; the two letters rate its two groups of cells.

Under the Gardner system most labs use, expansion runs from 1 (early blastocyst) to 6 (fully hatched out of its shell). The first letter grades the inner cell mass, which becomes the baby. The second grades the trophectoderm, the outer layer that becomes the placenta. A means many tightly packed cells, B fewer or looser, C very few. Some labs follow the ESHRE/ALPHA consensus and write 1, 2 and 3 (good, fair, poor) instead of letters.

03

What does a day 3 embryo grade mean?

On day 3, embryos are judged mainly by how many cells they have, how even those cells are, and how much fragmentation there is.

The ESHRE/ALPHA consensus expects about 4 cells on day 2 and about 8 on day 3. Fragments are small pieces of cell debris; a little is common. Labs turn this into a grade such as 1, 2 or 3, or A, B or C, but the scales differ. Check the key on your own report.

04

Does a better grade mean a better chance of pregnancy?

A better grade helps choose which embryo to transfer first, but it cannot promise a pregnancy, and a lower grade does not rule one out.

Grading is an embryologist's reading of how an embryo looks. It cannot see the chromosomes, which are the commonest reason a transfer fails. The 2025 ESHRE/ALPHA consensus says the outer layer and expansion matter most when ranking blastocysts. When two blastocysts look alike, labs usually choose a day 5 one before a day 6 one. Embryo testing (PGT) looks at chromosomes and may be considered in selected cases, discussed with you.

Honest note

Grading is subjective: the same embryo can be graded differently by two embryologists, or by two labs. It is a way to rank your embryos, not a forecast. That is why we never quote pregnancy chances by grade. (What the evidence says (ESHRE/ALPHA Istanbul consensus update, 2025))

Embryo testing (PGT)
05

Why were some of our embryos not frozen or used?

Some embryos stop growing (arrest) or do not reach a freezable blastocyst by day 6 or 7.

This is common. It usually reflects the egg or sperm the embryo came from, not something the lab did. Each lab sets its own freezing criteria, and your report should say why an embryo was not frozen. [CONFIRM: Jananam's freezing criteria] If embryos arrest at the same stage cycle after cycle, that pattern matters, and it is worth a doctor reading every report together.

Embryo freezingWhen to stop IVF
06

How many embryos will be transferred?

At Jananam, never more than two, and usually one.

Transferring more embryos raises the risk of twins, which carry more risk for mother and babies. A good-quality single embryo is the usual choice, and the rest can be frozen for later.

Honest note

We do not transfer more than two embryos under any circumstance. Single embryo transfer is preferred for tested euploid embryos at any age, and usually under 38 with a good blastocyst. (Dr Vani Sundarapandian)

Twins in IVF

Your report, line by line.

Common lines, as they are usually printed. Wording differs between labs; bring yours and we will read it with you.

The embryo's sex is never on the report, and is never disclosed.

COLUMNS
  • On the report
  • What it means

MII or M2

MEANING
A mature egg, ready to be fertilised.

MI or GV

MEANING
An immature egg. It usually cannot be fertilised.

2PN

MEANING
Normal fertilisation: one set of genes from the egg, one from the sperm.

1PN or 3PN

MEANING
Abnormal fertilisation. These embryos are usually not used.

8-cell, grade 1 (day 3)

MEANING
An embryo with about the expected number of cells for day 3, graded on the lab's own scale.

Fragmentation

MEANING
Small pieces of cell debris. A little is common; a lot lowers the grade.

Morula or compacting (day 4)

MEANING
The cells are pressing together before forming a blastocyst.

Expansion 1 to 6

MEANING
How far the blastocyst has grown, from early (1) to hatched (6).

ICM A, B or C

MEANING
Inner cell mass, the part that becomes the baby: many packed cells (A) to very few (C).

TE A, B or C

MEANING
Trophectoderm, the outer layer that becomes the placenta: many even cells (A) to very few (C).

Day 5, day 6 or day 7 blastocyst

MEANING
The day the embryo reached the blastocyst stage.

Arrested

MEANING
The embryo stopped growing.

Vitrified or cryopreserved

MEANING
Frozen by fast freezing, for a later transfer.

Assisted hatching

MEANING
A small opening made in the shell before transfer. The HFEA rates it grey (not enough good evidence). Jananam uses it for day-3 frozen embryos.

Time-lapse or morphokinetic score

MEANING
Growth recorded by camera in the incubator. The HFEA rates time-lapse black: no effect on the chance of a baby for most patients.

When to call us

When to call us

  • Your embryos stopped growing at the same stage in more than one cycle.
  • Every embryo was graded poor, or none reached blastocyst.
  • Eggs did not fertilise, or very few did.
  • Your embryos were called good, but transfers keep failing.
  • You would like someone to read your report with you, line by line.

Questions couples ask

Still unsure? Ask us on WhatsApp — a real person replies.

Is 4AA a good embryo?

A 4AA is an expanded blastocyst whose inner cell mass and outer layer both looked well formed; in most labs it is among the highest grades. It still cannot show the embryo's chromosomes, so it is not a guarantee.

Can a lower-grade embryo like 3BC lead to a pregnancy?

Yes, lower-grade embryos can and do lead to healthy pregnancies. The grade ranks your embryos against each other; it does not decide the outcome.

What is the difference between a day 5 and a day 6 blastocyst?

It is the day the embryo reached the blastocyst stage. When two look alike, labs usually choose the day 5 one first, but day 6 blastocysts are frozen and transferred too.

What does embryo arrest mean?

Embryo arrest means an embryo stopped growing before it could be transferred or frozen. One or two in a batch is common; the same pattern in every cycle needs a doctor to look at the eggs, the sperm and the protocol.

Why does my report use numbers when another clinic's used letters?

Labs use different grading scales. The Gardner system uses letters A, B and C; the ESHRE/ALPHA consensus uses 1, 2 and 3 for good, fair and poor.

Does the embryo report show whether it is a boy or a girl?

No. Sex selection is prohibited in India under the PCPNDT Act, 1994 and the ART Act, 2021, and an embryo's sex is never disclosed.

Dr Vani Sundarapandian

Reviewed by

Dr Vani Sundarapandian

MD, DGO, FRCOG (UK) · Founder, Jananam Fertility Centre

Written by the Jananam team with the embryology lab, and reviewed by Dr Vani Sundarapandian, MD, DGO, FRCOG (UK). Her position on embryo numbers is quoted in her own words.

Sources (4)
  1. ESHRE and ALPHA / Oxford Academic: The Istanbul consensus update: a revised ESHRE/ALPHA consensus on oocyte and embryo static and dynamic morphological assessment (Human Reproduction, 2025)
  2. HFEA (UK fertility regulator): Pre-implantation genetic testing for aneuploidy (PGT-A): add-on rating
  3. HFEA (UK fertility regulator): Time-lapse imaging and incubation: add-on rating
  4. HFEA (UK fertility regulator): Assisted hatching: add-on rating

Where to next.

More on this topic

Codes you don't understand? Send us the report.

Share your embryo report on WhatsApp and tell us what you want to know. A real person from Dr Vani's team replies during clinic hours (Mon to Sat, 9am to 5pm. Sunday closed.).

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